Hyaluronic acid (HA) ELISA Kit

Species Reactivity : General
UniProt : N/A
Abbreviation : HA
Alternative Names : N/A
Application : ELISA
Range : 1.56-100 ng/mL
Sensitivity : 0.78 ng/mL
Intra-AssayCV : ?4.3%
Inter-AssayCV : ?9.2%
Recovery : 1.02
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate HA in samples. An antibody specific for HA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for HA is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Hyaluronan (also called hyaluronic acid or hyaluronate) is an anionic, non-s µLfated glycosaminoglycan distributed widely thro µghout connective, epithelial, and neural tissues. It is unique among glycosaminoglycans in that it is uns µLfated, forms in the plasma membrane instead of the Golgi and can be very large with its molec µLar weight often reaching the millions.One of the chief components of the extracell µLar matrix, hyaluronan contributes significantly to cell proliferation and migration, and may also be involved in the progression of some malignant tumors. The average 70 kg (154 lbs) person has ro µghly 15 grams of hyaluronan in their body, one-third of which is turned over (degraded and syntheVolumed) every day. Hyaluronic acid is also a component of the group A streptococcal extracell µLar caps µLe, and is believed to play a role in vir µLence.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).