ELISA Kits Human Interleukin-6

Interleukin 6 (IL-6) is a 26 kDa glycoprotein composed of 184 amino acids. IL-6 is cytokine with multiple pleiotropic effect on inflammation, immune response, hematopoiesis and oncogenesis. IL-6 is expressed in many types of cells, such as adipocytes, fibroblasts, osteoblasts, endothelial cells, neurons, lymphocytes, monocytes, neutrophils etc. and its expression is dependent on cell activation. Circulating IL-6 levels in healthy people are very low, about 1 pg/ml, but during inflammation, IL-6 levels increase rapidly. Anti-inflammatory properties of IL-6 are mainly responsible for the antibacterial and beneficial regenerative processes. Pro-inflammatory properties of IL-6 negatively affect chronic inflammation and autoimmunity. Increased levels of IL-6 have been found in patients with atherosclerotic cardiovascular disease, insulin resistant, diabetes mellitus type 2, atopic asthma, rheumatoid arthritis but also in patients with advance stage cancer, blood circulating micrometastasis etc. IL-6 is an effective marker that might be able to predict upcoming respiratory failure with high accuracy and help physicians correctly allocate COVID-19 patients at an early stage. The Human Interleukin-6 ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human interleukin-6 (IL-6). The kit measures IL-6 in serum, plasma (EDTA, citrate, heparin), urine, bronchoalveolar lavage fluid (BALF), cerebrospinal fluid (CSF) and amniotic fluid. PRINCIPLE OF THE TEST In the BioVendor Human Interleukin-6 ELISA, standards and samples are incubated in microplate wells pre-coated with monoclonal anti-human IL-6 antibody. After 60 minutes incubation and washing, biotin labelled monoclonal anti-human IL-6 antibody is added and incubated for 60 minutes with captured IL-6. After another washing, streptavidin-HRP conjugate is added. After 30 minutes incubation and the last washing step, the remaining conjugate is allowed to react with the substrate solution (TMB). The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured. The absorbance is proportional to the concentration of IL-6. A standard curve is constructed by plotting absorbance values against concentrations of standards, and concentrations of unknown samples are determined using this standard curve. MATERIALS AND REAGENTS PERFORMANCE CHARACTERISTICS Sensitivity Limit of Detection (LOD) is calculated from the real IL-6 values in wells and is 0.32 pg/ml. *Dilution Buffer is pipetted into blank wells. • Limit of assay Samples with absorbances exceeding the absorbance of the highest standard should be measured again with higher dilution. The final concentration of samples calculated from the standard curve must be multiplied by the respective dilution factor. CE-IVD certificated