Species Reactivity : Human (Homo sapiens)
UniProt : N/A
Abbreviation : AHSG
Alternative Names : A2HS; AHS; FETUA; HSGA; Fetuin A; Alpha-2-HS-glycoprotein
Application : ELISA
Range : 0.78-50 ng/mL
Sensitivity : 0.37 ng/mL
Intra-AssayCV : ?6.2%
Inter-AssayCV : ?9.2%
Recovery : 0.92
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate AHSG in samples. An antibody specific for AHSG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAHSG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for AHSG is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AHSG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Alpha-2-HS-glycoprotein is a protein which is encoded by the AHSG gene.Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is syntheVolumed by hepatocytes. The AHSG molec µLe consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue.The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been post µLated that it participates in the development of the tissues. However, its exact significance is still obscure.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).