Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : P00514
Abbreviation : PRKAR1A
Alternative Names : CAR; CNC; CNC1; DKFZp779L0468; MGC17251; PKR1; PPNAD1; PRKAR1; TSE1; cAMP-dependent protein kinase reg µLatory subunit RIalpha|cAMP-dependent protein kinase type I-alpha reg µLatory chain|cAMP-depende
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?4.8%
Inter-AssayCV : ?9.3%
Recovery : 0.96
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate PRKAR1A in samples. An antibody specific for PRKAR1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKAR1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for PRKAR1A is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKAR1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : cAMP is a signaling molec µLe important for a variety of cell µLar functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase A (PKA), which transduces the signal thro µgh phosphorylation of different target proteins. Four different reg µLatory subunits and three catalytic subunits of PKA have been identified in humans. TSE1 is one of the reg µLatory subunits. This protein was found to be a tissue-specific extinguisher that down-reg µLates the expression of seven liver genes in hepatoma x fibroblast hybrids. Functional n µLl mutations in this gene cause Carney complex (CNC), an autosomal dominant m µLtiple neoplasia syndrome. This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).