Biotinilated

Species Reactivity : N/A
UniProt : N/A
Abbreviation : Biotin
Alternative Names : N/A
Application : ELISA
Range : 3.125-200 ng/mL
Sensitivity : 1.15 ng/mL
Intra-AssayCV : ?4.8%
Inter-AssayCV : ?10.6%
Recovery : 0.84
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Competitive ELISA
Analysis Method?? : Quantitive
Test principle : This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Biotin. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conj µgated Biotin and incubated. The competitive inhibition reaction is launched between with HRP labeled Biotin and unlabeled Biotin with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Biotin in the sample. The color development is stopped and the intensity of the color is measured.
Product Overview :
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).

Volume : 100 µL
Form : liquid
Conj µgation : Biotin
Stabilizer : 5mg/ml BSA ( IgG-Free, Protease-Free)
Storage : 0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6. -20? for 24 months , avoid repeated freezing and thawing.
Application : ELISA, WB, IHC/ICC
S µggested dilution : 1 : 100 – 1 : 1000 for enzyme immunohistochemistry on tissue sections 1 : 4000-1 : 80000 for ELISA and Western blotting with chromogenic substrates
Note : This product is for research use only, not for diagnostic or therapeutic use.

Volume : 100 µL
Form : liquid
Conj µgation : Biotin
Stabilizer : 5mg/ml BSA ( IgG-Free, Protease-Free)
Storage : 0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6. -20? for 24 months , avoid repeated freezing and thawing.
Application : ELISA, WB, IHC/ICC
S µggested dilution : 1 : 100 – 1 : 1000 for enzyme immunohistochemistry on tissue sections 1 : 4000-1 : 80000 for ELISA and Western blotting with chromogenic substrates
Note : This product is for research use only, not for diagnostic or therapeutic use.

Volume : 100 µL
Form : liquid
Conj µgation : Biotin
Stabilizer : 5mg/ml BSA ( IgG-Free, Protease-Free)
Storage : 0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6. -20? for 24 months , avoid repeated freezing and thawing.
Application : ELISA, WB, IHC/ICC
S µggested dilution : 1 : 100 – 1 : 1000 for enzyme immunohistochemistry on tissue sections 1 : 4000-1 : 80000 for ELISA and Western blotting with chromogenic substrates
Note : This product is for research use only, not for diagnostic or therapeutic use.

Volume : 100 µL
Form : liquid
Conj µgation : Biotin
Stabilizer : 5mg/ml BSA ( IgG-Free, Protease-Free)
Storage : 0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6. -20? for 24 months , avoid repeated freezing and thawing.
Application : ELISA, WB, IHC/ICC
S µggested dilution : 1 : 100 – 1 : 1000 for enzyme immunohistochemistry on tissue sections 1 : 4000-1 : 80000 for ELISA and Western blotting with chromogenic substrates
Note : This product is for research use only, not for diagnostic or therapeutic use.

Volume : 100 µL
Form : liquid
Conj µgation : Biotin
Stabilizer : 5mg/ml BSA ( IgG-Free, Protease-Free)
Storage : 0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6. -20? for 24 months , avoid repeated freezing and thawing.
Application : ELISA, WB, IHC/ICC
S µggested dilution : 1 : 100 – 1 : 1000 for enzyme immunohistochemistry on tissue sections 1 : 4000-1 : 80000 for ELISA and Western blotting with chromogenic substrates
Note : This product is for research use only, not for diagnostic or therapeutic use.

Volume : 100 µL
Form : liquid
Conj µgation : Biotin
Stabilizer : 5mg/ml BSA ( IgG-Free, Protease-Free)
Storage : 0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6. -20? for 24 months , avoid repeated freezing and thawing.
Application : ELISA, WB, IHC/ICC
S µggested dilution : 1 : 100 – 1 : 1000 for enzyme immunohistochemistry on tissue sections 1 : 4000-1 : 80000 for ELISA and Western blotting with chromogenic substrates
Note : This product is for research use only, not for diagnostic or therapeutic use.

Volume : 100 µL
Form : liquid
Conj µgation : Biotin
Stabilizer : 5mg/ml BSA ( IgG-Free, Protease-Free)
Storage : 0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6. -20? for 24 months , avoid repeated freezing and thawing.
Application : ELISA, WB, IHC/ICC
S µggested dilution : 1 : 100 – 1 : 1000 for enzyme immunohistochemistry on tissue sections 1 : 4000-1 : 80000 for ELISA and Western blotting with chromogenic substrates
Note : This product is for research use only, not for diagnostic or therapeutic use.

Volume : 100 µL
Form : liquid
Conj µgation : Biotin
Stabilizer : 5mg/ml BSA ( IgG-Free, Protease-Free)
Storage : 0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6. -20? for 24 months , avoid repeated freezing and thawing.
Application : ELISA, WB, IHC/ICC
S µggested dilution : 1 : 100 – 1 : 1000 for enzyme immunohistochemistry on tissue sections 1 : 4000-1 : 80000 for ELISA and Western blotting with chromogenic substrates
Note : This product is for research use only, not for diagnostic or therapeutic use.

Volume : 100 µL
Form : liquid
Conj µgation : Biotin
Stabilizer : 5mg/ml BSA ( IgG-Free, Protease-Free)
Storage : 0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6. -20? for 24 months , avoid repeated freezing and thawing.
Application : ELISA, WB, IHC/ICC
S µggested dilution : 1 : 100 – 1 : 1000 for enzyme immunohistochemistry on tissue sections 1 : 4000-1 : 80000 for ELISA and Western blotting with chromogenic substrates
Note : This product is for research use only, not for diagnostic or therapeutic use.

Volume : 100 µL
Form : liquid
Conj µgation : Biotin
Stabilizer : 5mg/ml BSA ( IgG-Free, Protease-Free)
Storage : 0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6. -20? for 24 months , avoid repeated freezing and thawing.
Application : ELISA, WB, IHC
S µggested dilution :
Note : This product is for research use only, not for diagnostic or therapeutic use.