Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : Q8MKH6
Abbreviation : TNNT1
Alternative Names : ANM; FLJ98147; MGC104241; STNT; TNT; TNTS; slow skeletal muscle troponin T|troponin T1; skeletal; slow|troponin-T1; skeletal; slow
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?4.7%
Inter-AssayCV : ?8.4%
Recovery : 0.92
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate TNNT1 in samples. An antibody specific for TNNT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNNT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for TNNT1 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : TNNT1 is a reg µLatory complex located on the thin filament of the sarcomere. This complex reg µLates striated muscle contraction in response to fluctuations in intracell µLar calcium concentration. This complex is composed of three subunits : troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromusc µLar disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, res µLting in death due to respiratory insufficiency, usually in the second year. M µLtiple transcript variants encoding different isoforms have been found for this gene.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).