Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : Q2TA35
Abbreviation : TM2D2
Alternative Names : BLP1; MGC125813; MGC125814; BBP-like protein 1|TM2 domain containing 2; isoform a
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?5.9%
Inter-AssayCV : ?10.3%
Recovery : 1.04
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate TM2D2 in samples. An antibody specific for TM2D2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTM2D2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for TM2D2 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM2D2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : TM2D2 contains a structural mod µLe related to that of the seven transmembrane domain G protein-coupled receptor superfamily. This protein has sequence and structural similarities to the beta-amyloid binding protein (BBP), but, unlike BBP, it does not reg µLate a response to beta-amyloid peptide. This protein may have reg µLatory roles in cell death or proliferation signal cascades. This gene has m µLtiple alternatively spliced transcript variants which encode two different isoforms.The deduced 214-amino acid protein contains several features of a G protein-coupled receptor, including 2 putative transmembrane domains in its C-terminal half, a DRY motif, and conserved cysteines and lysines. Northern blot analysis detected variable expression of a 1.35-kb BLP1 transcript in all human tissues examined.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).