Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : P11116
Abbreviation : LGALS1
Alternative Names : DKFZp686E23103; GAL1; GBP; S-Lac lectin 1|beta-galactoside-binding lectin L-14-I|galaptin|galectin 1|galectin-1|lactose-binding lectin 1
Application : ELISA
Range : 78.1-5000 pg/mL
Sensitivity : 27.5 pg/mL
Intra-AssayCV : ?4.6%
Inter-AssayCV : ?7.1%
Recovery : 0.9
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate LGALS1 in samples. An antibody specific for LGALS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGALS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for LGALS1 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Galectin-1 is one of the most important lectins to date participating in the malignant tumor development. With more and more data available on its expression in tumors it is already clear that this lectin is an important target for cancer diagnostics and treatment.Its first involvement in cancer was associated with cell adhesion. Later on the lectin was shown to induce apoptosis of activated T-cells, and experiments have confirmed that cancer cells express galectin-1 also to protect themselves from immune response. Latest finding is a participation of galectin-1 in tumor angiogenesis as an angiogenic factor. In most of the cases described, galectin-1 is up-reg µLated in tumors in comparison to normal tissue. Moreover, the presence of the lectin is associated with metastases and poor prognosis of disease outcome.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).