Species Reactivity : Chicken (Gallus)
UniProt : O42401
Abbreviation : MATN3
Alternative Names : DIPOA; EDM5; HOA; OADIP; OS2;
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?3.8%
Inter-AssayCV : ?7.8%
Recovery : 1.01
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate MATN3 in samples. An antibody specific for MATN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMATN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for MATN3 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MATN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Matrin 3(MATR3) is localized in the nuclear matrix. It may play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogran µLar network. Two transcript variants encoding the same protein have been identified for this gene.The deduced 845-amino acid protein has a calc µLated molec µLar mass of approximately 95 kD. Its primary structure consists of 33% charged residues and is generally hydrophilic. Like the lamins, matrin-3 has a positively charged N terminus that contains a large number of amino acids with free hydroxyl groups. A highly acidic domain near the C terminus, in which 32% of the amino acids are acidic, is a characteristic found in other nuclear proteins.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).