Species Reactivity : Guinea pig (Cavia)
UniProt : Q9Z1S9
Abbreviation : MC2R
Alternative Names : ACTHR; MGC125798; ACTH receptor|MC2 receptor|OTTHUMP00000162512|corticotropin receptor|melanocortin 2 receptor
Application : ELISA
Range : 15.6-1000 pg/mL
Sensitivity : 3.9 pg/mL
Intra-AssayCV : ?3.8%
Inter-AssayCV : ?7.8%
Recovery : 0.98
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate MC2R in samples. An antibody specific for MC2R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMC2R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for MC2R is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MC2R bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : The ACTH receptor is a type of melanocortin receptor (type 2), properly known as MC2, which is specific for ACTH.the ACTHR gene is expressed in normal corporal skin, scalp tissue, compound nevus tissue, basal cell carcinoma tissue, and c µLtured melanocytes; the CYP11A1 gene is expressed in squamous cell carcinoma tissue, normal facial skin, normal corporal skin, c µLtured melanocytes, and basal cell carcinoma tissue; the CYP17 gene is expressed in normal facial skin, corporal skin, c µLtured melanocytes, squamous cell carcinoma cells, and basal cell carcinoma cells; the CYP21A2 gene is expressed in melanoma tissue, squamous cell carcinoma tissue, basal cell carcinoma tissue, normal facial skin, and c µLtured melanocytes.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).