Species Reactivity : Human (Homo sapiens)
UniProt : Q9UIF7
Abbreviation : MUTYH
Alternative Names : RP4-534D1.2; CYP2C; MGC4416; MYH; A/G-specific adenine DNA glycosylase|OTTHUMP00000009098|OTTHUMP00000009102|mutY homolog
Application : ELISA
Range : 0.156-10 ng/mL
Sensitivity : 0.057 ng/mL
Intra-AssayCV : ?3.6%
Inter-AssayCV : ?6.7%
Recovery : 0.87
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate MUTYH in samples. An antibody specific for MUTYH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMUTYH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for MUTYH is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUTYH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : MUTYH glycosylase, involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. Mutations in this gene res µLt in heritable predisposition to colon and stomach cancer. M µLtiple transcript variants encoding different isoforms have been found for this gene. Mutations in the MUTYH gene cause an autosomal recessive form of familial adenomatous polyposis. Polyps caused by mutated M
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).