Species Reactivity : Horse (Equus caballus; Equine)
UniProt : Q8MKG2
Abbreviation : SCGB1A1
Alternative Names : CC10; CC16; CCSP; µgB; Uteroglobin (Clara-cell specific 10-kD protein)
Application : ELISA
Range : 1.56-100 ng/mL
Sensitivity : 0.8 ng/mL
Intra-AssayCV : ?6.4%
Inter-AssayCV : ?10.1%
Recovery : 0.95
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate SCGB1A1 in samples. An antibody specific for SCGB1A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCGB1A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for SCGB1A1 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCGB1A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Clara Cell Protein 16 concentrations have been determined in both serum and bronchoalveolar lavage fluid in numerous studies since 1994. In serum, its increase is associated with age, asbestos, nitrogen chloride and ozone exposure, sarcoidosis and high PEEP ventilation. Decreased serum CC16 levels are found after p µLmonary resection, in silica-exposed workers, smokers and in asthma. Decreased CC16 concentrations were also found in the amniotic fluid of fetuses suffering from p µLmonary hypoplasia caused by various mechanisms (diaphragmatic hernia, diabetic fetopathy, Turner and Down syndrome). In pleural effusions, the CC16 concentration appears to be associated with its diffusion from the lung as evidenced by high CC16 levels in cardiac pleural congestion.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).