Species Reactivity : Human (Homo sapiens)
UniProt : N/A
Abbreviation : CA50
Alternative Names : N/A
Application : ELISA
Range : 3.12-200 ng/mL
Sensitivity : 1.29 ng/mL
Intra-AssayCV : ?5.1%
Inter-AssayCV : ?9.8%
Recovery : 0.93
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate CA50 in samples. An antibody specific for CA50 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCA50 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for CA50 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CA50 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Carbohydrate antigen 50 (CA 50) is a tumor marker that increases in many malignancies, especially in carcinoma of the digestive tract. False-positive res µLts occur in benign liver disease.Significant correlations with some liver tests were found, especially transaminases, and at lower degrees with cholestatic parameters. Despite the previously reported relation with cholestasis, especially in biliary diseases, the CA 50 serum levels of the authors' cirrhotic patients appeared to be more closely related to cytolysis, according to the res µLts of several statistical tests, including m µLtivariate analysis.Because of the percentage and the levels of the abnormal res µLts, this antigen cannot be used as a tumor marker in cirrhotic patients. Cytolysis seems to have a pathogenetic role in the increase of CA 50, at least in cirrhosis.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).