Species Reactivity : Human (Homo sapiens)
UniProt : O43852
Abbreviation : CALU
Alternative Names : FLJ90608; crocalbin-like protein|m µLtiple EF-hand protein
Application : ELISA
Range : 0.625-40 ng/mL
Sensitivity : 0.263 ng/mL
Intra-AssayCV : ?4.6%
Inter-AssayCV : ?7.4%
Recovery : 0.96
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate CALU in samples. An antibody specific for CALU has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCALU present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for CALU is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CALU bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Calumenin (CALU) is a calcium-binding protein localized in the endoplasmic retic µLum (ER) and is involved in such ER functions as protein folding and sorting. Calumenin is a member of the EF-hand superfamily in the ER and Golgi apparatus named CERC. CERC is an acronym for its family members Cab-45, retic µLocalbin, Erc-55, and calumenin. The CALU gene encodes a deduced 315-amino acid protein containing 6 EF-hand motifs, 1 potential N-glycosylation site, and a C-terminal ER retention signal. The human and mouse CALU proteins are 98% identical. CALU mRNA is ubiquitously expressed in human tissues and maps to 7q32.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).