Species Reactivity : Human (Homo sapiens)
UniProt : Q99417
Abbreviation : MYCBP
Alternative Names : AMY-1; FLJ41056; associate of myc-1
Application : ELISA
Range : 0.156-10 ng/mL
Sensitivity : 0.061 ng/mL
Intra-AssayCV : ?4.2%
Inter-AssayCV : ?8.2%
Recovery : 1.05
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate MYCBP in samples. An antibody specific for MYCBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYCBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for MYCBP is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYCBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : The MYCBP gene encodes a protein that binds to the N-terminal region of MYC and stim µLates the activation of E box-dependent transcription by MYC.The MYC protooncogene appears to play key roles in cell proliferation, differentiation, transformation, and apoptosis. A variety of functions of the MYC gene product are attributable to protein-protein interactions with various cell µLar factors. AMY1 was found to bind in vitro and in vivo to the reg µLatory subunit II-binding region of AKAP1 and S-AKAP84, a splicing variant of AKAP149 expressed in the testis. AMY1 was expressed postmeiotically in the testis, as was also S-AKAP84. AMY1 was localized in the mitochondria of HeLa and sperm in association with AKAP149 and S-AKAP84, respectively. These res µLts s µggested that AMY1 plays a role in spermatogenesis.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).