Species Reactivity : Human (Homo sapiens)
UniProt : P09619
Abbreviation : PDGFRB
Alternative Names : CD140B; JTK12; PDGFR; PDGFR1; CD140 antigen-like family member B|PDGF-R-beta|platelet-derived growth factor receptor beta
Application : ELISA
Range : 0.156-10 ng/mL
Sensitivity : 0.054 ng/mL
Intra-AssayCV : ?5.3%
Inter-AssayCV : ?9.6%
Recovery : 1.08
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate PDGFRB in samples. An antibody specific for PDGFRB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDGFRB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for PDGFRB is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGFRB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : PDGFRb is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for gran µLocyte-macrophage colony-stim µLating factor and macrophage-colony stim µLating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, res µLts in chronic myeloproliferative disorder with eosinophilia.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).