Species Reactivity : Human (Homo sapiens)
UniProt : P98170
Abbreviation : XIAP
Alternative Names : RP1-315G1.5; API3; BIRC4; ILP1; MIHA; XLP2; OTTHUMP00000196392|apoptosis inhibitor 3|bac µLoviral IAP repeat-containing 4|bac µLoviral IAP repeat-containing protein 4
Application : ELISA
Range : 0.156-10 ng/mL
Sensitivity : 0.056 ng/mL
Intra-AssayCV : ?4.9%
Inter-AssayCV : ?9.8%
Recovery : 1.03
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate XIAP in samples. An antibody specific for XIAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXIAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for XIAP is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XIAP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : X-linked Inhibitor of Apoptosis Protein (XIAP) is a member of the Inhibitor of apoptosis family of proteins (IAP). IAPs were initially identified in bac µLoviruses, but XIAP is one of the homologous proteins found in mammals. It is so called because it was first discovered by a 273 base pair site on the X chromosome.The protein is also called human IAP-like Protein (hILP), because it is not as well conserved as the human IAPS : hIAP-1 and hIAP-2. XIAP is the most potent human IAP protein currently identified.XIAP, like the rest of the IAP family, has two major structural elements. Firstly, there is the bac µLoviral IAP repeat (BIR) domain consisting of approximately 70 amino acids. Secondly, there is a zinc-binding domain, or a “carboxy-terminal RING Finger”.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).