Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : O02703
Abbreviation : BAX
Alternative Names : BCL2L4; apoptosis reg µLator BAX
Application : ELISA
Range : 0.312-20 ng/mL
Sensitivity : 0.124 ng/mL
Intra-AssayCV : ?4.0%
Inter-AssayCV : ?7.8%
Recovery : 0.99
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate BAX in samples. An antibody specific for BAX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBAX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for BAX is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BAX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Bax (Bcl-2 associated x protein) is an apoptosis reg µLating protein of the Bcl-2 family. The ratio of Bax to Bcl-2 determines survival or death following an apoptotic stim µLus. Bax is implicated in many cancers, uterine leiomyomas, and brain dysmorphology. Bax is a 21 kDa protein that accelerates apoptosis by binding to, and antagonizing the death repressor activity of Bcl-2 in vivo.Bax normally resides in the cytoplasm, but following an apoptotic stim µLi, Bax undergoes a conformational change and translocates to mitochondrial membranes, where it inserts and mediates the release of cytochrome c from the intermembrane space into the cytosol, in this manner activating caspase-3 and perpetuating the apoptotic cascade.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).