Volume : 50 µg
Clone Number :
Aliases : LEP antibody; OB antibody; OBS antibody; Leptin antibody; Obesity factor antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Bos taurus (Bovine)
UniProt ID : P50595
Immunogen : Recombinant Bovine Leptin protein (22-167AA)
Raised in : Rabbit
Species Reactivity : Bovine
Tested Applications :
Background : Key player in the reg µLation of energy balance and body weight control. Once released into the circ µLation, has central and peripheral effects by binding LEPR, found in many tissues, which res µLts in the activation of several major signaling pathways (By similarity). In the hypothalamus, acts as an appetite-reg µLating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also reg µLates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, reg µLates pancreatic beta-cell function and ins µLin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity (By similarity). In the arcuate nucleus of the hypothalamus, activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides (By similarity). In addition to its known satiety inducing effect, has a mod µLatory role in nutrient absorption. In the intestine, reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38, PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption (By similarity). Acts as a growth factor on certain tissues, thro µgh the activation of different signaling pathways increases expression of genes involved in cell cycle reg µLation such as CCND1 via JAK2-STAT3 pathway, or VEGFA, via MAPK1/3 and PI3K-AKT1 pathways (By similarity). May also play an apoptotic role via JAK2-STAT3 pathway and up-reg µLation of BIRC5 expression. Pro-angiogenic, has mitogenic activity on vasc µLar endothelial cells and plays a role in matrix remodeling by reg µLating the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). In innate immunity, mod µLates the activity and function of neutrophils by increasing chemotaxis and the secretion of oxygen radicals. Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators. Increases cytotoxic ability of NK cells. Plays a pro-inflammatory role, in synergy with IL1B, by inducing NOS2 wich promotes the production of IL6, IL8 and Prostaglandin E2, thro µgh a signaling pathway that involves JAK2, PI3K, MAP2K1/MEK1 and MAPK14/p38 (By similarity). In adaptive immunity, promotes the switch of memory T-cells towards T helper-1 cell immune responses (By similarity). Increases CD4(+)CD25(-) T-cell proliferation and reduces autophagy during TCR (T-cell receptor) stim µLation, thro µgh MTOR signaling pathway activation and BCL2 up-reg µLation (By similarity).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : LEP
Research Areas : Neuroscience; Cancer; Cardiovasc µLar; Metabolism; Signal transduction; Stem cells
