FITC

Volume : 50 µg
Clone Number :
Aliases : annexin 14 antibody; Annexin A10 antibody; Annexin-10 antibody; Annexin-14 antibody; ANX 14 antibody; ANX10_HUMAN antibody; ANX14 antibody; ANXA 10 antibody; ANXA10 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q9UJ72
Immunogen : Recombinant Human Annexin A10 protein (138-222AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background : calcium ion binding
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : ANXA10
Research Areas : Signal transduction

Volume : 50 µg
Clone Number :
Aliases : Anxa1 antibody; Anx1 antibody; Lpc-1 antibody; Lpc1Annexin A1 antibody; Annexin I antibody; Annexin-1 antibody; Calpactin II antibody; Calpactin-2 antibody; Chromobindin-9 antibody; Lipocortin I antibody; Phospholipase A2 inhibitory protein antibody; p35 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Mus musc µLus (Mouse)
UniProt ID : P10107
Immunogen : Recombinant Mouse Annexin A1 protein (2-346AA)
Raised in : Rabbit
Species Reactivity : Mouse
Tested Applications :
Background : Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein reg µLates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : Anxa1
Research Areas : Signal transduction

Product Details
CD3 is a protein complex composed of five peptide chains (CD3γ, CD3δ, CD3ε, CD3η, CD3ζ), and mainly expressed in all mature T cells, NKT cells, and some thymocytes. It is a member of the immunoglob µLin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation by binding to the T-cell receptor (TCR) . Trans-1A5 monoclonal antibody specifically recognizes CD3ε chain in CD3/TCR complex, which can be used for the activation and expansion of T cells. After purification of Trans-1A5 monoclonal antibody by affinity chromatography, the antibodies were conj µgated with fluorescein FITC under optimal conditions and used for flow cytometric detection of CD3 on cell surface.

Storage
at 2-8°C for one year


Shipping
ice bag (4℃)



Product Contents

Component HF151-01 HF151-02
Anti-Human CD3, FITC 25 tests 50 tests

Volume : 50 µg
Clone Number :
Aliases : Alanyl (membrane) aminopeptidase antibody; Alanyl aminopeptidase antibody; Aminopeptidase M antibody; Aminopeptidase N antibody; AMPN_HUMAN antibody; ANPEP antibody; AP M antibody; AP N antibody; AP-M antibody; AP-N antibody; APN antibody; CD 13 antibody; CD13 antibody; CD13 antigen antibody; gp150 antibody; hAPN antibody; LAP 1 antibody; LAP1 antibody; Microsomal aminopeptidase antibody; Myeloid plasma membrane glycoprotein CD13 antibody; p150 antibody; PEPN antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P15144
Immunogen : Recombinant Human Aminopeptidase N protein (34-219AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background : Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of reg µLatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. Found to cleave antigen peptides bound to major histocompatibility complex class II molec µLes of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a reg µLator of IL-8 bioavailability in the endometrium, and therefore may contribute to the reg µLation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : ANPEP
Research Areas : Immunology; Stem cells

Volume : 50 µg
Clone Number :
Aliases : Acidic leucine-rich nuclear phosphoprotein 32 family member D antibody; AN32D_HUMAN antibody; ANP32D antibody; Phosphoprotein 32-related protein 2 antibody; Tumorigenic protein pp32r2 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : O95626
Immunogen : Recombinant Human Acidic leucine-rich nuclear phosphoprotein 32 family member D protein (1-131AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background :
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : ANP32D
Research Areas : Others

Volume : 50 µg
Clone Number :
Aliases : 2900059G15Rik antibody; AA407480 antibody; Ano6 antibody; ANO6_HUMAN antibody; Anoctamin 6 antibody; Anoctamin-6 antibody; AW554778 antibody; BDPLT7 antibody; F730003B03Rik antibody; MGC104751 antibody; SCTS antibody; TMEM16F antibody; Transmembrane protein 16F antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q4KMQ2
Immunogen : Recombinant Human Anoctamin-6 protein (12-100AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background : Small-conductance calcium-activated nonselective cation (SCAN) channel which acts as a reg µLator of phospholipid scrambling in platelets and osteoblasts. Phospholipid scrambling res µLts in surface exposure of phosphatidylserine which in platelets is essential to trigger the clotting system whereas in osteoblasts is essential for the deposition of hydroxyapatite during bone mineralization. Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide (By similarity). Can generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat T lymphocytes.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : ANO6
Research Areas : Others

Volume : 50 µg
Clone Number :
Aliases : ANO4 antibody; TMEM16DAnoctamin-4 antibody; Transmembrane protein 16D antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q32M45
Immunogen : Recombinant Human Anoctamin-4 protein (1-200AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background : Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide. Does not exhibit calcium-activated chloride channel (CaCC) activity.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : ANO4
Research Areas : Others

Volume : 50 µg
Clone Number :
Aliases : Ano2 antibody; ANO2_HUMAN antibody; Anoctamin 2 antibody; Anoctamin-2 antibody; C12orf3 antibody; Chromosome 12 open reading frame 3 antibody; DKFZp434P102 antibody; Transmembrane protein 16B (eight membrane-spanning domains) antibody; Transmembrane protein 16B antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q9NQ90
Immunogen : Recombinant Human Anoctamin-2 protein (1-83AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background : Calcium-activated chloride channel (CaCC) which may play a role in olfactory signal transduction. Odorant molec µLes bind to odor-sensing receptors (OSRs), leading to an increase in calcium entry that activates CaCC current which amplifies the depolarization of the OSR cells, ANO2 seems to be the underlying chloride channel involved in this process. May mediate light perception amplification in retina.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : ANO2
Research Areas : Neuroscience

Volume : 50 µg
Clone Number :
Aliases : ANO 1 antibody; ANO1 antibody; ANO1_HUMAN antibody; Anoctamin 1 antibody; Anoctamin 1 calcium activated chloride channel antibody; Anoctamin-1 antibody; Anoctamin1 antibody; Ca2+ activated Cl- channel antibody; Calcium Activated Chloride Channel antibody; Discovered on gastrointestinal stromal tumors protein 1 antibody; DOG 1 antibody; DOG1 antibody; FLJ10261 antibody; Membrane protein antibody; Oral cancer overexpressed 2 antibody; Oral cancer overexpressed protein 2 antibody; ORAOV 2 antibody; ORAOV2 antibody; TAOS 2 antibody; TAOS2 antibody; TMEM 16A antibody; TMEM16A antibody; Transmembrane protein 16A (eight membrane spanning domains) antibody; Transmembrane protein 16A antibody; Tumor amplified and overexpressed sequence 2 antibody; Tumor-amplified and overexpressed sequence 2 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q5XXA6
Immunogen : Recombinant Human Anoctamin-1 protein (1-151AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background : Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stim µLated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : ANO1
Research Areas : Tags & Cell Markers

In healthy cells, phosphatidylserine (PS) only distributes in the inner layer of the cell membrane lipid bilayer. At the early stage of apoptosis, PS is turned from the inside of the lipid membrane to the outside. Annexin V, a Ca2+ dependent phospholipid binding protein with a molec µLar weight of 35-36 kD, has a high affinity to PS and can be used to detect to detect the externalization of PS. Annexin V is recognized as one of the sensitive indicators of early apoptosis. Apoptosis can be detected by fluorescence microscopy or flow cytometry using Annexin V probe conj µgated to FITC (a green fluorescent). This kit also includes propidium iodide (PI), a dye for nucleic acid that cannot penetrate the intact cell membrane of healthy or early apoptotic cells. PI can penetrate the membrane of late apoptotic and necrotic cells, and stain these cells with red fluorescence. Using both Annexin V and PI, this kit can used to distinguish cells at different apoptotic periods, i.e. live cells (Annexin V-/PI-), early apoptotic cells (Annexin V+/PI-), and late apoptotic/dead cells (Annexin V+/PI+).
Component
A211-01 (50 rxn)
A211-02 (100 rxn)
Annexin V-FITC
250 µl
500 µl
PI Staining Solution
250 µl
500 µl
1× Binding Buffer 25 ml 2 × 25 ml
Store all components at 4-8℃ and protect from light.
The Annexin V-FITC can be stored at -20℃ for longer use.
Fig. 1. Detection of TRAIL-induced apoptosis of Jurkat cells by flow cytometry (A) 0 ng/ml. (B) 0.5 ng/ml. (C) 1.5 ng/ml. (D) 4.5 ng/ml. (E) 13.5 ng/ml. (F) Jurkat cells were induced apoptosis for 3 hours by 13.5 ng/ml TRAIL and then treated with 10 μg unlabeled Annexin V for 10 min, followed by Annexin V-FITC/PI staining. Unlabeled Annexin V, competiting with Annexin V-FITC, was used to confirm the specificity of staining

Volume : 50 µg
Clone Number :
Aliases : Annexin A5 antibody; Annexin V antibody; Annexin-5 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Cynops pyrrhogaster
UniProt ID : P70075
Immunogen : Recombinant Cynops pyrrhogaster Annexin A5 protein (1-323AA)
Raised in : Rabbit
Species Reactivity : Cynops pyrrhogaster
Tested Applications :
Background : Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names :
Research Areas : Others