Volume : 50 µg
Clone Number :
Aliases : Akt2 antibody; AKT2_HUMAN antibody; HIHGHH antibody; Murine thymoma viral (v-akt) homolog 2 antibody; murine thymoma viral (v-akt) homolog-2 antibody; Oncogene AKT2 protein kinase B beta antibody; PKB antibody; PKB beta antibody; PKBB antibody; PKBBETA antibody; PRKBB antibody; Protein kinase Akt 2 antibody; Protein kinase Akt-2 antibody; Protein kinase B beta antibody; RAC beta antibody; rac protein kinase beta antibody; RAC-BETA antibody; RAC-beta serine/threonine-protein kinase antibody; RAC-PK-beta antibody; RACbeta antibody; v akt murine thymoma viral oncogene homolog 2 antibody; V-AKT murine thymoma viral oncogene homolog 2 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P31751
Immunogen : Recombinant Human RAC-beta serine/threonine-protein kinase protein (2-481AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background : AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1 AKT2 and AKT3) called the AKT kinase, and which reg µLate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated thro µgh serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificityhas been reported. AKT is responsible of the reg µLation of glucose uptake by mediating ins µLin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively mod µLates its phosphatase activity preventing dephosphorylation of the ins µLin receptor and the attenuation of ins µLin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for ins µLin-stim µLated glucose transport. AKT reg µLates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', res µLting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also tho µght to be one mechanism by which cell proliferation is driven. AKT reg µLates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). Phosphorylation of 'Ser-83' decreases MAP3K5 kinase activity stim µLated by oxidative stress and thereby prevents apoptosis. AKT mediates ins µLin-stim µLated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization. In partic µLar, FOXO1 is phosphorylated at 'Thr-24', 'Ser-256' and 'Ser-319'. FOXO3 and FOXO4 are phosphorylated on equivalent sites. AKT has an important role in the reg µLation of NF-kappa-B-dependent gene transcription and positively reg µLates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT phosphorylates 'Ser-454' on ATP citrate lyase (ACLY), thereby potentially reg µLating ACLY activity and fatty acid synthesis. Activates the 3B isoform ofcyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of 'Ser-273', res µLting in reduced cyclic AMP levels and inhibition of lipolysis. Phosphorylates PIKFYVE on 'Ser-318', which res µLts in increased PI3P-5 activity. The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the reg µLation cell proliferation and cell growth. AKT plays a role as key mod µLator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during ad µLt neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), ins µLin and ins µLin-like growth factor I (IGF-I). AKT mediates the antiapoptotic effects of IGF-I. Essential for the SPATA13-mediated reg µLation of cell migration and adhesion assembly and disassembly. May be involved in the reg µLation of the placental development. Ref.10 Ref.20 One of the few specific substrates of AKT2 identified recently is PITX2. Phosphorylation of PITX2 impairs its association with the CCND1 mRNA-stabilizing complex thus shortening the half-life of CCND1. AKT2 seems also to be the principal isoform responsibleof the reg µLation of glucose uptake. Phosphorylates C2CD5 on 'Ser-197' during ins µLin-stim µLated adipocytes. AKT2 is also specifically involved in skeletal muscle differentiation, one of its substrates in this process being ANKRD2. Down-reg µLation by RNA interference reduces the expression of the phosphorylated form of BAD, res µLting in the induction of caspase-dependent apoptosis. Phosphorylates CLK2 on 'Thr-343'.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : AKT2
Research Areas : Cancer; Cardiovasc µLar; Metabolism; Signal transduction