In healthy cells, phosphatidylserine (PS) only distributes in the inner layer of the cell membrane lipid bilayer. At the early stage of apoptosis, PS is turned from the inside of the lipid membrane to the outside. Annexin V, a Ca2+ dependent phospholipid binding protein with a molec µLar weight of 35-36 kD, has a high affinity to PS and can be used to detect to detect the externalization of PS. Annexin V is recognized as one of the sensitive indicators of early apoptosis. Apoptosis can be detected by fluorescence microscopy or flow cytometry using Annexin V probe conj µgated to FITC (a green fluorescent). This kit also includes propidium iodide (PI), a dye for nucleic acid that cannot penetrate the intact cell membrane of healthy or early apoptotic cells. PI can penetrate the membrane of late apoptotic and necrotic cells, and stain these cells with red fluorescence. Using both Annexin V and PI, this kit can used to distinguish cells at different apoptotic periods, i.e. live cells (Annexin V-/PI-), early apoptotic cells (Annexin V+/PI-), and late apoptotic/dead cells (Annexin V+/PI+).
Component
A211-01 (50 rxn)
A211-02 (100 rxn)
Annexin V-FITC
250 µl
500 µl
PI Staining Solution
250 µl
500 µl
1× Binding Buffer 25 ml 2 × 25 ml
Store all components at 4-8℃ and protect from light.
The Annexin V-FITC can be stored at -20℃ for longer use.
Fig. 1. Detection of TRAIL-induced apoptosis of Jurkat cells by flow cytometry (A) 0 ng/ml. (B) 0.5 ng/ml. (C) 1.5 ng/ml. (D) 4.5 ng/ml. (E) 13.5 ng/ml. (F) Jurkat cells were induced apoptosis for 3 hours by 13.5 ng/ml TRAIL and then treated with 10 μg unlabeled Annexin V for 10 min, followed by Annexin V-FITC/PI staining. Unlabeled Annexin V, competiting with Annexin V-FITC, was used to confirm the specificity of staining

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : ANXA5
GeneID : 308
Uniprot : P08758
Specificity : Annexin V Antibody detects endogenous levels of total Annexin V.
Immunogen : A syntheVolumed peptide derived from human Annexin V, corresponding to a region within C-terminal amino acids.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Mouse,Rat
Prediction : Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
Application : WB,IHC,ELISA(peptide)
Dilution : WB 1 : 500-1 : 2000, IHC 1 : 50-1 : 200, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2838542
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : DF6580-BP
Des# Blocking Peptide : Annexin V Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : ANXA4
GeneID : 307
Uniprot : P09525
Specificity : Annexin IV Antibody detects endogenous levels of total Annexin IV.
Immunogen : A syntheVolumed peptide derived from human Annexin IV, corresponding to a region within the internal amino acids.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Mouse
Prediction : Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
Application : WB,ELISA(peptide)
Dilution : WB 1 : 500-1 : 2000, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2843950
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : AF7586-BP
Des# Blocking Peptide : Annexin IV Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : ANXA3
GeneID : 306
Uniprot : P12429
Specificity : Annexin III Antibody detects endogenous levels of total Annexin III.
Immunogen : A syntheVolumed peptide derived from human Annexin III, corresponding to a region within N-terminal amino acids.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Mouse,Rat
Prediction : Bovine,Horse,Sheep,Rabbit,Dog,Xenopus
Application : WB,IHC,ELISA(peptide)
Dilution : WB 1 : 500-1 : 2000, IHC 1 : 50-1 : 200, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2837645
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : AF5159-BP
Des# Blocking Peptide : Annexin III Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 50 µg
Protein Type : Other proteins
Product Grade : For Research Use.
Tag : None
Species : Human
Expressed In : E.coli
Note : Usually in stock. Smaller quantities and volume discounts are available. For further information, please contact us.

Volume : 50 µg
Protein Type : Other proteins
Product Grade : For Research Use.
Tag : None
Species : Human
Expressed In : E.coli
Note : Usually in stock. Smaller quantities and volume discounts are available. For further information, please contact us.

Volume : 50 µg
Protein Type : Other proteins
Product Grade : For Research Use.
Tag : C-6His
Species : Human
Expressed In : E.coli
Note : Usually in stock. Smaller quantities and volume discounts are available. For further information, please contact us.

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : ANXA6
GeneID : 309
Uniprot : P08133
Specificity : Annexin A6 Antibody detects endogenous levels of total Annexin A6.
Immunogen : A syntheVolumed peptide derived from human Annexin A6, corresponding to a region within N-terminal amino acids.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Mouse,Rat
Prediction : Pig,Bovine,Sheep,Dog
Application : WB,IHC,IF/ICC,ELISA(peptide)
Dilution : WB : 1 : 500-1 : 3000, IHC : 1 : 50-1 : 200, IF/ICC : 1 : 100-1 : 500, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2833266
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : AF0116-BP
Des# Blocking Peptide : Annexin A6 Blocking peptide
Blocking Peptide Price : $549 CAD

Volume : 50 µg
Protein Type : Other proteins
Product Grade : For Research Use.
Tag : None
Species : Human
Expressed In : E.coli
Note : Usually in stock. Smaller quantities and volume discounts are available. For further information, please contact us.

Volume : 50 µg
Clone Number :
Aliases : Annexin A5 antibody; Annexin V antibody; Annexin-5 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Cynops pyrrhogaster
UniProt ID : P70075
Immunogen : Recombinant Cynops pyrrhogaster Annexin A5 protein (1-323AA)
Raised in : Rabbit
Species Reactivity : Cynops pyrrhogaster
Tested Applications : ELISA
Background : Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names :
Research Areas : Others

Volume : 50 µg
Clone Number :
Aliases : Annexin A5 antibody; Annexin V antibody; Annexin-5 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Cynops pyrrhogaster
UniProt ID : P70075
Immunogen : Recombinant Cynops pyrrhogaster Annexin A5 protein (1-323AA)
Raised in : Rabbit
Species Reactivity : Cynops pyrrhogaster
Tested Applications :
Background : Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names :
Research Areas : Others