Species Reactivity : Human (Homo sapiens)
UniProt : Q9Y5H1
Abbreviation : PCDHGA2
Alternative Names : PCDH-GAMMA-A2;
Application : ELISA
Range : 0.156-10 ng/mL
Sensitivity : 0.063 ng/mL
Intra-AssayCV : ?4.2%
Inter-AssayCV : ?7.9%
Recovery : 1.02
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate PCDHGA2 in samples. An antibody specific for PCDHGA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCDHGA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for PCDHGA2 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCDHGA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Cadherins are calcium-dependent cell-cell adhesion molec µLes, and protocadherins constitute a subfamily of nonclassic cadherins. PCDHGA2 is 1 of 22 tandemly arranged genes within the PCDHG gene cluster on chromosome 5q31. The 22 PCDHG genes function as 'variable' exons that are individually spliced to a downstream constant region to form distinct PCDHG transcripts. The variable PCDHG exons encode the extracell µLar and transmembrane domains of the protocadherin protein, and the common region encodes the intracell µLar domain. For further information on the PCDHG. By genomic sequence analysis, Wu et al. (2001) mapped the PCDHGA2 gene to 5q31 between the PCDHGA1 gene and the PCDHGA3 gene. They localized the mouse PCDHG genes to chromosome 18c.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).