Species Reactivity : Human (Homo sapiens)
UniProt : P17252
Abbreviation : PRKCA
Alternative Names : AAG6; MGC129900; MGC129901; PKC-alpha; PKCA; PRKACA; aging-associated gene 6|protein kinase C alpha type
Application : ELISA
Range : 31.25-2000 pg/mL
Sensitivity : 12.2 pg/mL
Intra-AssayCV : ?4.8%
Inter-AssayCV : ?6.9%
Recovery : 0.97
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate PRKCA in samples. An antibody specific for PRKCA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKCA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for PRKCA is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKCA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : PRKCA, refers to both a human gene and the protein that is encoded by it. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cell µLar signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cell µLar processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).