Species Reactivity : Guinea pig (Cavia)
UniProt : P11578
Abbreviation : ITGAM
Alternative Names : CD11B; CR3A; MAC-1; MAC1A; MGC117044; MO1A; SLEB6; antigen CD11b (p170)|integrin alpha M|macrophage antigen alpha polypeptide|neutrophil adherence receptor alpha-M subunit
Application : ELISA
Range : 0.156-10 ng/mL
Sensitivity : 0.057 ng/mL
Intra-AssayCV : ?7.3%
Inter-AssayCV : ?7.9%
Recovery : 1.04
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate ITGAM in samples. An antibody specific for ITGAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyITGAM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for ITGAM is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ITGAM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (alpha M beta 2) molec µLe, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3). ITGAM is also known as CR3A, and cluster of differentiation molec µLe 11B (CD11B). The second chain of alpha beta 2 is the common integrin beta 2 subunit known as CD18, and integrin alpha M beta 2 thus belongs to the beta 2 subfamily (or leukocyte) integrins alpha M beta 2 is expressed on the surface of many leukocytes involved in the innate immune system, including monocytes, gran µLocytes, macrophages, and natural killer cells.It mediates inflammation by reg µLating leukocyte adhesion and migration and has been implicated in several immune processes such as phagocytosis, cell-mediated cytotoxicity, chemotaxis and cell µLar activation.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).