Species Reactivity : Human (Homo sapiens)
UniProt : P33908
Abbreviation : MAN1A1
Alternative Names : HUMM3; HUMM9; MAN9; Man9-mannosidase|alpha-1;2-mannosidase IA|mannosyl-oligosaccharide 1;2-alpha-mannosidase IA
Application : ELISA
Range : 0.156-10 ng/mL
Sensitivity : 0.057 ng/mL
Intra-AssayCV : ?3.5%
Inter-AssayCV : ?7.6%
Recovery : 0.8
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate MAN1A1 in samples. An antibody specific for MAN1A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAN1A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for MAN1A1 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAN1A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : MAN1A1 encodes a class I mammalian Golgi 12-mannosidase which is a type II transmembrane protein. This protein catalyzes the removal of 3 distinct mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides and belongs to family 47 of glycosyl hydrolases. Using an oligonucleotide probe derived from a pig liver Man(9)-mannosidase-specific cDNA template, Bause et al. (1993) isolated Man(9)-mannosidase from a human kidney cDNA library. The f µLl-length cDNA predicted a 625-amino acid protein with a calc µLated molec µLar mass of 71 kD. Man(9)-mannosidase is a type II transmembrane protein with a short cytoplasmic polypeptide tail, a single transmembrane domain acting as a noncleavable signal sequence, a large luminal catalytic domain, and 3 potential N-glycosylation sites.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).