Elisa 96 Wells

Volume : 96 Test
Species : Bovine
Uniprot ID : P11052
Short name : GM-CSF
Alias : GM-CSF|Gran µLocyte Macrophage Colony Stim µLating Factor|GMCSF|CSF2
Sensitivity : 9.375pg/ml
Range : 15.625-1000pg/ml
Detection method : Sandwich ELISA, Double Antibody
Storage_tempeRature : 2-8 °C for 6 months
Research Area : Immunology, Cardiovasc µLar, Signal Transduction

Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : P79334
Abbreviation : PYGM
Alternative Names : muscle glycogen phosphorylase|myophosphorylase
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?5.6%
Inter-AssayCV : ?7.9%
Recovery : 1.01
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate PYGM in samples. An antibody specific for PYGM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPYGM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for PYGM is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYGM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Mutations in the muscle isoform of glycogen phosphorylase (PYGM) are associated with McArdle disease (glycogen storage disease type V). More than 65 mutations in the PYGM gene that lead to McArdle disease have been identified to date.Gautron et al. (1987) isolated muscle phosphorylase cDNA clones from a human cDNA library. Northern blot experiments revealed 1 specific mRNA of 3.4 kb found uniquely in tissues expressing muscle phosphorylase. The muscle glycogen phosphorylase protein comprises 842 amino acids (Kubisch et al., 1998).Burke et al. (1987) determined the intron/exon structure of the PYGM gene. Kubisch et al. (1998) provided a revised genomic structure for the PYGM gene, which contains 20 exons.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).

Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : Q0VCM4
Abbreviation : PYGL
Alternative Names : GSD6; liver glycogen phosphorylase
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?6.1%
Inter-AssayCV : ?8.4%
Recovery : 1.04
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate PYGL in samples. An antibody specific for PYGL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPYGL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for PYGL is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYGL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : PYGL is a homodimeric protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further reg µLated by m µLtiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, or Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and res µLts in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).

Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : Q3B7M9
Abbreviation : PYGB
Alternative Names : MGC9213; brain glycogen phosphorylase|glycogen phosphorylase B
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?4.8%
Inter-AssayCV : ?8.6%
Recovery : 0.96
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate PYGB in samples. An antibody specific for PYGB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPYGB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for PYGB is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYGB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : PYGB is a new isozyme of glycogen phosphorylase (14-D-glucan : orthosphosphate D-glucosyltransferase; EC 2.4.1.1) from a cDNA library prepared from a human brain astrocytoma cell line. Blot-hybridization analysis showed that the message is preferentially expressed in human brain, but is also found at a low level in human fetal liver and ad µLt liver and muscle tissues. The protein sequence deduced from the nucleotide sequence was 862 amino acids long compared with 846 and 841 amino acids for the liver (PYGL) and muscle (PYGM) isozymes, respectively. The greater length of brain phosphorylase was found to be due entirely to an extension at the c-terminal portion of the protein. Muscle and brain isozymes shared greater similarities with each other than either did with the liver sequence.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).

Volume : 96-test Kit. Other Volumes are also available. Please contact us.
Assay Type : Sandwich
Detection Range : 0.156-10ng/mL
Sensitivity : 0.092ng/mL
Species : Bovine
GeneName : GLYCAM1
Alternative Names : GlyCAM-128 kDa milk glycoprotein PP3,Lactophorin,Proteose-peptone component 3,PP3
Uniport : P80195

Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt :
Abbreviation : GSH
Alternative Names : N/A
Application :
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV :
Inter-AssayCV :
Recovery :
Sample Type : Serum, Plasma, Other biological fluids
Detection Method :
Analysis Method?? :
Test principle :
Product Overview :
Stability :

Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : Q29RP9
Abbreviation : QRSL1
Alternative Names : DKFZp564C1278; FLJ10989; FLJ12189; FLJ13447; GatA;
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?6.4%
Inter-AssayCV : ?11.5%
Recovery : 1.12
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate QRSL1 in samples. An antibody specific for QRSL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQRSL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for QRSL1 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QRSL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : QRSL1 belongs to the amidase family, similar to glutaminyl-tRNA synthetase. Glutaminyl-tRNA synthetase is a class Ic synthetase and shows several similarities with glutamyl-tRNA synthetase concerning structure and catalytic properties. It is an alpha2 dimer. Glutaminyl-tRNA synthetase is a relatively rare synthetase, found in the cytosolic compartment of eukaryotes, in Escherichia coli and a number of other Gram-negative bacteria, and in Deinococcus radiodurans. In contrast, the pathway to Gln-tRNA in mitochondria, Archaea, Gram-positive bacteria, and a number of other lineages is by misacylation with Glu followed by transamidation to correct the aminoacylation to Gln. A stable glutaminly-adenylate analog, which inhibits GlnRS with a Ki of 1.32 microM, was syntheVolumed and cocrystallized with GlnRS and tRNA2Gln.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).

Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : Q3MHH4
Abbreviation : QARS
Alternative Names : GLNRS; PRO2195; glutamine tRNA ligase|glutamine-tRNA synthetase
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?4.5%
Inter-AssayCV : ?8.1%
Recovery : 0.99
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate QARS in samples. An antibody specific for QARS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQARS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for QARS is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QARS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are tho µght to be among the first proteins that appeared in evolution. In metazoans, 9 aminoacyl-tRNA synthetases specific for glutamine (gln), glutamic acid (glu), and 7 other amino acids are associated within a m µLtienzyme complex. Altho µgh present in eukaryotes, glutaminyl-tRNA synthetase (QARS) is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln). Glutaminyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).

Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : Q0V8G3
Abbreviation : QPCTL
Alternative Names : FLJ20084; glutaminyl cyclase-like
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?3.8%
Inter-AssayCV : ?7.1%
Recovery : 1.03
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate QPCTL in samples. An antibody specific for QPCTL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQPCTL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for QPCTL is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QPCTL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : QPCTL, also termed Iso-glutaminyl cyclase catalyzes the intramolec µLar cyclization of N-terminal glutamine residues into pyroglutamic acid with liberation of ammonia and the intramolec µLar cyclization of N-terminal glutamate residues into pyroglutamic acid with liberation of water. Glutaminyl cyclase (QPCT) catalyzes the intramolec µLar cyclization of N-terminal glutamine residues into pyroglutamic acid liberating ammonia. In contrast, the physiological function of the plant QC is less clear. In case of the enzyme from C. papaya, a role in the plant defence against pathogenic microorganisms was s µggested. Putative QCs from other plants were identified by sequence comparisons. The physiological function of these enzymes, however, is still ambiguous.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).

Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : Q28120
Abbreviation : QPCT
Alternative Names : GCT; QC; glutaminyl cyclase
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?5.2%
Inter-AssayCV : ?7.9%
Recovery : 1.03
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate QPCT in samples. An antibody specific for QPCT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQPCT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for QPCT is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QPCT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : QPCT encodes human pituitary glutaminyl cyclase, which is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides. The amino acid sequence of this enzyme is 86% identical to that of bovine glutaminyl cyclase. The deduced 361-amino acid protein has a calc µLated molec µLar mass of about 41 kD. It contains an N-terminal signal peptide region, several glycosylation and phosphorylation sites, and 2 cysteine residues conserved between the bovine and human enzymes. QPCT shares 86% overall sequence identity with the bovine homolog.Glutaminyl cyclase expression was upreg µLated in the cortices of individuals with Alzheimer disease and correlated with the appearance of pE-modified amyloid beta.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).

Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : Q0P5J0
Abbreviation : QRICH1
Alternative Names : FLJ20259; MGC131838; OTTHUMP00000210560
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?6.7%
Inter-AssayCV : ?9.2%
Recovery : 1.1
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate QRICH1 in samples. An antibody specific for QRICH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQRICH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for QRICH1 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QRICH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : QRICH1 contains 1 CARD domain. Glutamine is one of the 20 amino acids encoded by the standard genetic code. It is not an essential amino acid. Its side-chain is an amide formed by replacing the side-chain hydroxyl of glutamic acid with an amine functional group. Therefore, it can be considered the amide of glutamic acid. Caspase activation and recruitment domains (CARDs), are interaction motifs found in a wide array of proteins, typically those involved in processes relating to inflammation and apoptosis. These domains mediate the formation of larger protein complexes via direct interactions between individual CARDs. CARD domains are found on a strikingly wide range of proteins, including helicases, kinases, mitochondrial proteins, caspases, and other cytoplasmic factors.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).