Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : Q27967
Abbreviation : SPP2
Alternative Names : SPP24; OTTHUMP00000065382|secreted phosphoprotein 24
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?4.2%
Inter-AssayCV : ?6.9%
Recovery : 0.98
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate SPP2 in samples. An antibody specific for SPP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for SPP2 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that reg µLates diverse biologic processes. SGPP2 catalyzes the degradation of S1P.The deduced 399-amino acid protein has a calc µLated molec µLar mass of 44.7 kD and shares 39.3% amino acid identity with SGPP1. SGPP2 has an N-terminal hydrophobic region, 3 conserved phosphatase-family motifs, and is predicted to be an integral membrane protein with as many as 9 membrane-spanning segments. Immunofluorescence microscopy showed a retic µLar staining pattern in the perinuclear and cytosolic regions of HEK293 cells, and SGPP2 colocalized with an endoplasmic retic µLum antibody. Northern blot analysis of human tissues detected a 5.1-kb transcript with high expression in kidney and heart, followed by brain, colon, lung, and small intestine.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).