Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : A7YVH9
Abbreviation : PYROXD1
Alternative Names : FLJ22028;
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?5.2%
Inter-AssayCV : ?8.1%
Recovery : 0.98
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate PYROXD1 in samples. An antibody specific for PYROXD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPYROXD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for PYROXD1 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYROXD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : PYROXD1 Belongs to the class-I pyridine nucleotide-dis µLfide oxidoreductase family. In biochemistry, an oxidoreductase is an enzyme that catalyzes the transfer of electrons from one molec µLe (the reductant, also called the hydrogen or electron donor) to another (the oxidant, also called the hydrogen or electron acceptor). This group of enzymes usually utilizes NADP or NAD as cofactors. Proper names of oxidoreductases are formed as "donor : acceptor oxidoreductase"; however, other names are much more common. The common name is "donor dehydrogenase" when possible, such as glyceraldehyde-3-phosphate dehydrogenase for the second reaction above. Common names are also sometimes formed as "acceptor reductase", such as NAD+ reductase. "Donor oxidase" is a special case where O2 is the acceptor.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).