Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : Q9XS47
Abbreviation : PGF
Alternative Names : D12S1900; PGFL; PLGF; PlGF-2; SHGC-10760; placenta growth factor|placental growth factor; vasc µLar endothelial growth factor-related protein|placental growth factor-like
Application : ELISA
Range : 15.6-1000 pg/mL
Sensitivity : 5.9 pg/mL
Intra-AssayCV : ?5.9%
Inter-AssayCV : ?8.6%
Recovery : 1.03
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate PGF in samples. An antibody specific for PGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for PGF is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Placental growth factor (PLGF) is a member of the VEGF (vasc µLar endothelial growth factor) sub-family - a key molec µLe in angiogenesis and vasc µLogenesis, in partic µLar during embryogenesis. The main source of PLGF during pregnancy is the placental trophoblast. PLGF is also expressed in many other tissues, including the villous trophoblast. PLGF is a dimeric glycoprotein that, like VEGF, can bind with high affinity to the tyrosine kinase receptor vasc µLar endothelial growth factor receptor 1 (VEGFR-1/Flt-1). PLGF/VEGF heterodimers have been isolated and shown to bind also to the VEGFR-2/KDR/Flk-1 receptor the major mediator of VEGF activities.PLGF shares approximately 42% amino acid (aa) sequence identity with VEGF (53% within the PDGF region), and the two proteins share significant structural similarity.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).