Species Reactivity : Pig (Sus scrofa; Porcine)
UniProt : Q5XW65
Abbreviation : PLUNC
Alternative Names : LPLUNC3; LUNX; NASG; SPLUNC1; SPURT; bA49G10.5; OTTHUMP00000030627|ligand-binding protein RYA3|nasopharyngeal carcinoma-related protein|secretory protein in upper respiratory tracts|tracheal epithel
Application : ELISA
Range : 15.6-1000 pg/mL
Sensitivity : 6.9 pg/mL
Intra-AssayCV : ?4.5%
Inter-AssayCV : ?8.3%
Recovery : 1
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate PLUNC in samples. An antibody specific for PLUNC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLUNC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for PLUNC is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLUNC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : PLUNC is the human homolog of murine plunc, and like the mouse gene, is specifically expressed in the upper airways and nasopharyngeal regions. The exact biological function of this gene is not known, however, it has been s µggested to be involved in inflammatory responses to irritants in the upper airways. It may also serve as a potential molec µLar marker for detection of micrometastasis in non-small-cell lung cancer. M µLtiple transcript variants res µLting from alternative splicing in the 3' UTR have been detected, but the f µLl-length nature of only two is known.The cDNA encodes a leucine-rich protein of 256 amino acids that is 72% identical to the murine protein. within the human PLUNC family, amino acid sequence identity is low, ranging from 16 to 28%.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).