Species Reactivity : Canine (Canis familiaris; Dog)
UniProt : P01353
Abbreviation : GAST
Alternative Names : GAS;
Application : ELISA
Range : 12.35-1000 pg/mL
Sensitivity : 4.95 pg/mL
Intra-AssayCV : ?4.8%
Inter-AssayCV : ?7.9%
Recovery : 0.93
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate GAST in samples. An antibody specific for GAST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGAST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for GAST is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAST bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Gastrin is a major physiological reg µLator of gastric acid secretion. It also has an important trophic or growth-promoting influence on the gastric mucosa. Gastrin is syntheVolumed in G cells, which are located in gastric pits, primarily in the antrum region of the stomach and binds receptors found predominantly on parietal and enterochromaffin-like cells.In humans, gastrin is a hormone that stim µLates secretion of gastric acid by the parietal cells of the stomach. It is released by G cells in the stomach and duodenum.Gastrin is a linear peptide hormone produced by G cells of the duodenum and in the pyloric antrum of the stomach. In the Zollinger-Ellison syndrome, gastrin is produced at excessive levels, often by a gastrinoma of the antrum or the pancreas.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).