Species Reactivity : Chicken (Gallus)
UniProt : Q5F3E8
Abbreviation : TNFAIP1
Alternative Names : B12; B61; EDP1; MGC2317; tumor necrosis factor; alpha-induced protein 1
Application : ELISA
Range : 15.6-1000 pg/mL
Sensitivity : 6.5 pg/mL
Intra-AssayCV : ?4.7%
Inter-AssayCV : ?8.9%
Recovery : 0.98
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate TNFAIP1 in samples. An antibody specific for TNFAIP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFAIP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for TNFAIP1 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFAIP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : TNFAIP1 was identified as a gene whose expression can be induced by the tumor necrosis factor alpha (TNF) in umbilical vein endothelial cells. Studies of a similar gene in mouse s µggest that the expression of this gene is developmentally reg µLated in a tissue-specific manner. Wolf et al. (1992) characterized a novel cDNA by differential screening of a tumor necrosis factor-alpha (TNFA)-stim µLated umbilical vein endothelial cell library.The protein product is involved in the primary response of the endothelium to TNF. The 3.5-kb transcript was found to be expressed in a developmentally reg µLated, tissue-specific manner. The gene, present in single copy, was located in the 17q22-q23 region by in situ hybridization.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).