Species Reactivity : Chicken (Gallus)
UniProt : Q5ZIA2
Abbreviation : MAP7
Alternative Names : E-MAP-115; EMAP115; dJ325F22.2 (microtub µLe-associated protein 7 (EMAP115; E-MAP-115))
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?3.4%
Inter-AssayCV : ?7.2%
Recovery : 0.89
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate MAP7 in samples. An antibody specific for MAP7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAP7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for MAP7 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Ensconsin is a microtub µLe-associated protein that is predominantly expressed in cells of epithelial origin. Microtub µLe-associated proteins are tho µght to be involved in microtub µLe dynamics, which is essential for cell polarization and differentiation. This protein has been shown to be able to stabilize microtub µLes, and may serve to mod µLate microtub µLe functions. Studies of the related mouse protein also s µggested an essential role in microtub µLe function required for spermatogenesis. Expressed in the skin and cells of epithelial origin. Predominantly expressed in the suprabasal layers of the normal epidermis and relatively abundant in squamous cell carcinomas but barely detectable in basal cell carcinomas.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).