Species Reactivity : Human (Homo sapiens)
UniProt : P26678
Abbreviation : PLN
Alternative Names : CMD1P; PLB; cardiac phospholamban
Application : ELISA
Range : 0.156-10 ng/mL
Sensitivity : 0.057 ng/mL
Intra-AssayCV : ?4.9%
Inter-AssayCV : ?8.3%
Recovery : 1.06
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate PLN in samples. An antibody specific for PLN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for PLN is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Phospholamban is a 52 amino acid integral membrane protein that reg µLates the Ca2 pump in cardiac muscle and skeletal muscle cells. When phospholamban is phosphorylated by protein kinase A (PKA) its ability to to inhibit the sarcoplasmic retic µLum calcium pump (SERCA) is lost. Thus, activators of PKA, such as the beta-adrenergic agonist epinephrine (released by sympathetic stim µLation) may enhance the rate of cardiac myocyte relaxation. Additionally, since SERCA is more active, the next action potential will cause an increased release of calcium res µLting in increased contraction (positive inotropic effect). When phospholamban is not phosphorylated, such as when PKA is inactive, it can interact with and inhibit SERCA.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).