Species Reactivity : Human (Homo sapiens)
UniProt : P82251
Abbreviation : SLC7A9
Alternative Names : BAT1; CSNU3; FLJ94301; B(0;+)-type amino acid transporter 1|bo;+ amino acid transporter|glycoprotein-associated amino acid transporter b0;+AT1|solute carrier family 7; member 9
Application : ELISA
Range : 1.56-100 ng/mL
Sensitivity : 0.55 ng/mL
Intra-AssayCV : ?4.3%
Inter-AssayCV : ?7.5%
Recovery : 0.97
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate SLC7A9 in samples. An antibody specific for SLC7A9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC7A9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for SLC7A9 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC7A9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : The SLC7A9 cDNA is polyadenylated and contains an open reading frame encoding a 487-amino acid protein. The protein, designated b(0,+)AT for b(0,+) amino acid transporter, belongs to a family of light subunits of amino acid transporters expressed in kidney, liver, small intestine, and placenta. Northern blot analysis revealed that the SLC7A9 gene was expressed as an approximately 1.9-kb transcript in these tissues. The tissue distribution of b(0,+)AT was consistent with that of a renal basic amino acid transporter (see SLC3A1 or rBAT) light subunit. As expected, b(0,+)AT bro µght rBAT to the plasma membrane in cotransfected COS cells. In contrast, transfection of rBAT alone res µLted in the blockage of the expressed protein in the endoplasmic retic µLum.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).