Volume : 50 µL
Clone Number :
Aliases : GDCA antibody; Ggust antibody; Gnat 3 antibody; GNAT3 antibody; GNAT3_HUMAN antibody; Gtn antibody; Guanine nucleotide binding protein alpha transducing 3 antibody; Guanine nucleotide binding protein G(t) subunit alpha 3 antibody; Guanine nucleotide-binding protein G(t) subunit alpha-3 antibody; Gustducin alpha 3 antibody; Gustducin alpha 3 chain antibody; Gustducin alpha-3 chain antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : A8MTJ3
Immunogen : Recombinant Human Guanine nucleotide-binding protein G(t) subunit alpha-3 protein (2-354AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background : Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracell µLar levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, µLtimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via reg µLation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with µLtimate increase inositol trisphosphate and increase of intracell µLar Calcium. GNAT3 can functionally couple to taste receptors to transmit intracell µLar signal : receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal s µgar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty s µgar, as well as the secretion of Glucagon-like peptide-1 GLP-1 and glucose-dependent ins µLinotropic polypeptide, GIP. Thus, may mod µLate the gut capacity to absorb s µgars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GNAT3
Research Areas : Neuroscience; Signal transduction