Volume : 50 µg
Clone Number :
Aliases : IGHMImmunoglob µLin heavy constant mu antibody; Ig mu chain C region antibody; Ig mu chain C region BOT antibody; Ig mu chain C region GAL antibody; Ig mu chain C region OU antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P01871
Immunogen : Recombinant Human Immunoglob µLin heavy constant mu protein (45-178AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background : Constant region of immunoglob µLin heavy chains. Immunoglob µLins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglob µLins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglob µLins-secreting plasma cells. Secreted immunoglob µLins mediate the effector phase of humoral immunity, which res µLts in the elimination of bound antigens (PubMed : 22158414, PubMed : 20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglob µLin has two antigen binding sites with remarkable affinity for a partic µLar antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a partic µLar antigen (PubMed : 17576170, PubMed : 20176268). IgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cell µLar waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The membrane-bound form is found in the majority of normal B-cells alongside with IgD. Membrane-bound IgM induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. It may cause death of cells by apoptosis. It is also found in soluble form, which represents about 30% of the total serum immunoglob µLins where it is found almost exclusively as a homopentamer. After the antigen binds to the B-cell receptor, the secreted form is secreted in large amounts (PubMed : 3137579, PubMed : 16895553).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : IGHM
Research Areas : Immunology