Species Reactivity : Human (Homo sapiens)
UniProt : P02679
Abbreviation : FGG
Alternative Names : Fibrinogen; gamma chain|fibrinogen; gamma polypeptide
Application : ELISA
Range : 6.25-400 ng/mL
Sensitivity : 2.52 ng/mL
Intra-AssayCV : ?4.7%
Inter-AssayCV : ?8.6%
Recovery : 0.99
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate FGG in samples. An antibody specific for FGG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFGG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for FGG is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Fibrinogen gamma chain is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vasc µLar injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin reg µLate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing res µLts in two transcript variants encoding different isoforms.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).