Species Reactivity : Human (Homo sapiens)
UniProt : Q9NZH8
Abbreviation : IL1F9
Alternative Names : IL-1F9; IL-1H1; IL-1RP2; IL1E; IL1H1; IL1RP2; IL-1 related protein 2|IL-1(EPSILON)|IL-1-epsilon|interleukin 1-related protein 2|interleukin-1 epsilon|interleukin-1 homolog 1
Application : ELISA
Range : 15.6-1000 pg/mL
Sensitivity : 5.9 pg/mL
Intra-AssayCV : ?3.8%
Inter-AssayCV : ?6.4%
Recovery : 0.92
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate IL1F9 in samples. An antibody specific for IL1F9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL1F9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for IL1F9 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL1F9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : IL1F9 encoded by this gene is a member of the interleukin 1 cytokine family. The activity of this cytokine is mediated by interleukin 1 receptor-like 2, and is specifically inhibited by interleukin 1 family, member 5 . Interferon-gamma, tumor necrosis factor-alpha and interleukin 1 beta (IL1B) are reported to stim µLate the expression of this cytokine in keratinocytes. The expression of this cytokine in keratinocytes can also be induced by a contact hypersensitivity reaction or herpes simplex virus infection. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.Highly expressed in tissues containing epithelial cells : skin, lung, stomach and esophagus. In skin is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Up-reg µLated in lesional psoriasis skin.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).