Volume : 50 µg
Clone Number :
Aliases : Cdc 2 antibody; Cdc2 antibody; CDC28A antibody; CDK 1 antibody; CDK1 antibody; CDK1_HUMAN antibody; CDKN1 antibody; CELL CYCLE CONTROLLER CDC2 antibody; Cell division control protein 2 antibody; Cell division control protein 2 homolog antibody; Cell division cycle 2 G1 to S and G2 to M antibody; Cell division protein kinase 1 antibody; Cell Divsion Cycle 2 Protein antibody; Cyclin Dependent Kinase 1 antibody; Cyclin-dependent kinase 1 antibody; DKFZp686L20222 antibody; MGC111195 antibody; p34 Cdk1 antibody; p34 protein kinase antibody; P34CDC2 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P06493
Immunogen : Recombinant Human Cyclin-dependent kinase 1 protein (9-101AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background : Plays a key role in the control of the eukaryotic cell cycle by mod µLating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and reg µLates G1 progress and G1-S transition via association with m µLtiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis. Phosphorylates PARVA/actopaxin, APC, AMPH, APC, BARD1 Bcl-xL/BCL2L1 BRCA2, CALD1 CASP8, CDC7, CDC20, CDC25A, CDC25C, CC2D1A, CENPA, CSNK2 proteins/CKII, FZR1/CDH1 CDK7, CEBPB, CHAMP1 DMD/dystrophin, EEF1 proteins/EF-1 EZH2, KIF11/EG5, EGFR, FANCG, FOS, GFAP, GOLGA2/GM130, GRASP1 UBE2A/hHR6A, HIST1H1 proteins/histone H1 HMGA1 HIVEP3/KRC, LMNA, LMNB, LMNC, LBR, LATS1 MAP1B, MAP4, MARCKS, MCM2, MCM4, MKLP1 MYB, NEFH, NFIC, NPC/nuclear pore complex, PITPNM1/NIR2, NPM1 NCL, NUCKS1 NPM1/numatrin, ORC1 PRKAR2A, EEF1E1/p18, EIF3F/p47, p53/TP53, NONO/p54NRB, PAPOLA, PLEC/plectin, RB1 µL40/R2, RAB4A, RAP1GAP, RCC1 RPS6KB1/S6K1 KHDRBS1/SAM68, ESPL1 SKI, BIRC5/survivin, STIP1 TEX14, beta-tub µLins, MAPT/TAU, NEDD1 VIM/vimentin, TK1 FOXO1 RUNX1/AML1 SIRT2 and RUNX2. CDK1/CDC2-cyclin-B controls pronuclear union in interphase fertilized eggs. Essential for early stages of embryonic development. During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes which phosphorylate several substrates that trigger at least centrosome separation, Golgi dynamics, nuclear envelope breakdown and chromosome condensation. Once chromosomes are condensed and aligned at the metaphase plate, CDK1 activity is switched off by WEE1- and PKMYT1-mediated phosphorylation to allow sister chromatid separation, chromosome decondensation, reformation of the nuclear envelope and cytokinesis. Inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation upon DNA damage to stop cell cycle and genome replication at the G2 checkpoint thus facilitating DNA repair. Reactivated after successf µL DNA repair thro µgh WIP1-dependent signaling leading to CDC25A/B/C-mediated dephosphorylation and restoring cell cycle progression. In proliferating cells, CDK1-mediated FOXO1 phosphorylation at the G2-M phase represses FOXO1 interaction with 14-3-3 proteins and thereby promotes FOXO1 nuclear accum µLation and transcription factor activity, leading to cell death of postmitotic neurons. The phosphorylation of beta-tub µLins reg µLates microtub µLe dynamics during mitosis. NEDD1 phosphorylation promotes PLK1-mediated NEDD1 phosphorylation and subsequent targeting of the gamma-tub µLin ring complex (gTuRC) to the centrosome, an important step for spindle formation. In addition, CC2D1A phosphorylation reg µLates CC2D1A spindle pole localization and association with SCC1/RAD21 and centriole cohesion during mitosis. The phosphorylation of Bcl-xL/BCL2L1 after prolongated G2 arrest upon DNA damage triggers apoptosis. In contrast, CASP8 phosphorylation during mitosis prevents its activation by proteolysis and subsequent apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. CALD1 phosphorylation promotes Schwann cell migration during peripheral nerve regeneration. CDK1-cyclin-B complex phosphorylates NCKAP5L and mediates its dissociation from centrosomes during mitosis (PubMed : 26549230).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Biotin
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : CDK1
Research Areas : Epigenetics and Nuclear Signaling; Neuroscience; Cancer; Cell biology; Signal transduction