Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : P80457
Abbreviation : XDH
Alternative Names : XO; XOR; xanthene dehydrogenase|xanthine oxidase|xanthine oxidoreductase
Application : ELISA
Range : 0.781-50 ng/mL
Sensitivity : 0.27 ng/mL
Intra-AssayCV : ?5.6%
Inter-AssayCV : ?9.8%
Recovery : 1.07
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate XDH in samples. An antibody specific for XDH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXDH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for XDH is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XDH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The enzyme is a homodimer. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible s µLfhydryl oxidation or by irreversible proteolytic modification. The three substrates of this enzyme are xanthine, NAD+, and H2O, whereas its three products are urate, NADH, and H+.This enzyme belongs to the family of oxidoreductases, to be specific, those acting on CH or CH2 group with NAD+ or NADP+ as acceptor.Defects in xanthine dehydrogenase cause xanthinuria, may contribute to ad µLt respiratory stress syndrome, and may potentiate influenza infection thro µgh an oxygen metabolite-dependent mechanism.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).