Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : Q27956
Abbreviation : LIF
Alternative Names : CDF; DIA; HILDA; D factor|cholinergic differentiation factor|differentiation inhibitory activity|differentiation stim µLating factor
Application : ELISA
Range : 31.25-2000 pg/mL
Sensitivity : 12.9 pg/mL
Intra-AssayCV : ?7.4%
Inter-AssayCV : ?9.2%
Recovery : 1.01
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate LIF in samples. An antibody specific for LIF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLIF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for LIF is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LIF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Leukemia inhibitory factor, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukaemic cells. Other properties attributed to the cytokine include : the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. LIF is normally expressed in the trophectoderm of the developing embryo, with its receptor LIFR expressed thro µghout the inner cell mass. As embryonic stem cells are derived from the inner cell mass at the blastocyst stage, removing them from the inner cell mass also removes their source of LIF.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).