Species Reactivity : Chicken (Gallus)
UniProt : O42281
Abbreviation : PRPH2
Alternative Names : AOFMD; AVMD; CACD2; PRPH; RDS; RP7; TSPAN22; rd2; peripherin 2|peripherin 2; homolog of mouse|peripherin; photoreceptor type|retinal peripherin|tetraspanin-22
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?5.6%
Inter-AssayCV : ?8.7%
Recovery : 0.98
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate PRPH2 in samples. An antibody specific for PRPH2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRPH2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for PRPH2 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRPH2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Peripherin 2 is a cell surface glycoprotein found in the outer segment of both rod and cone photoreceptor cells. It is located in the rim regions of the flattened disks that contain rhodopsin, which is the protein that is responsible for initiation of visual phototransduction upon reception of light. Peripherin 2 may function as an adhesion molec µLe involved in stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. This protein is essential for disk morphogenesis. Defects in this gene are associated with both central and peripheral retinal degenerations. Some of the various phenotypically different disorders are autosomal dominant retinitis pigmentosa, progressive mac µLar degeneration, mac µLar dystrophy and retinitis pigmentosa digenic.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).