Volume : 50 µL
Clone Number :
Aliases : BING 2 antibody; BING2 antibody; CENP-C binding protein antibody; DAP 6 antibody; DAP6 antibody; Daxx antibody; DAXX_HUMAN antibody; Death associated protein 6 antibody; Death domain associated protein 6 antibody; Death domain associated protein antibody; Death domain-associated protein 6 antibody; EAP 1 antibody; EAP1 antibody; ETS1 associated protein 1 antibody; ETS1-associated protein 1 antibody; Fas binding protein antibody; Fas death domain associated protein antibody; Fas death domain-associated protein antibody; hDaxx antibody; MGC126245 antibody; MGC126246 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q9UER7
Immunogen : Recombinant Human Death domain-associated protein 6 protein (77-289AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background : Transcription corepressor known to repress transcriptional potential of several sumoylated transcription factors. Down-reg µLates basal and activated transcription. Its transcription repressor activity is mod µLated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies thro µgh interactions with MCSR1 and PML, respectively. Seems to reg µLate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Inhibits transcriptional activation of PAX3 and ETS1 thro µgh direct protein-protein interactions. Mod µLates PAX5 activity; the function seems to involve CREBBP. Acts as an adapter protein in a MDM2-DAXX-USP7 complex by reg µLating the RING-finger E3 ligase MDM2 ubiquitination activity. Under non-stress condition, in association with the deubiquitinating USP7, prevents MDM2 self-ubiquitination and enhances the intrinsic E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and subsequent proteasomal degradation. Upon DNA damage, its association with MDM2 and USP7 is disrupted, res µLting in increased MDM2 autoubiquitination and consequently, MDM2 degradation, which leads to TP53 stabilization. Acts as histone chaperone that facilitates deposition of histone H3.3. Acts as targeting component of the chromatin remodeling complex ATRX : DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Does not affect the ATPase activity of ATRX but alleviates its transcription repression activity. Upon neuronal activation associates with reg µLatory elements of selected immediate early genes where it promotes deposition of histone H3.3 which may be linked to transcriptional induction of these genes. Required for the recruitment of histone H3.3 : H4 dimers to PML-nuclear bodies (PML-NBs); the process is independent of ATRX and facilitated by ASF1A; PML-NBs are s µggested to function as reg µLatory sites for the incorporation of newly syntheVolumed histone H3.3 into chromatin. In case of overexpression of centromeric histone variant CENPA (as found in various tumors) is involved in its mislocalization to chromosomes; the ectopic localization involves a heterotypic tetramer containing CENPA, and histones H3.3 and H4 and decreases binding of CTCF to chromatin. Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Shows restriction activity towards human cytomegalovirus (HCMV). Plays a role as a positive reg µLator of the heat shock transcription factor HSF1 activity during the stress protein response (PubMed : 15016915).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : DAXX
Research Areas : Epigenetics and Nuclear Signaling; Cell biology; Microbiology
