Volume : 50 µL
Clone Number :
Aliases : hns antibody; bglY antibody; cur antibody; drdX antibody; hnsA antibody; msyA antibody; osmZ antibody; pilG antibody; topS antibody; b1237 antibody; JW1225 antibody; DNA-binding protein H-NS antibody; Heat-stable nucleoid-structuring protein antibody; Histone-like protein HLP-II antibody; Protein B1 antibody; Protein H1 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Escherichia coli (strain K12)
UniProt ID : P0ACF8
Immunogen : Recombinant Escherichia coli DNA-binding protein H-NS protein (39-137AA)
Raised in : Rabbit
Species Reactivity : Escherichia coli
Tested Applications :
Background : A DNA-binding protein implicated in transcriptional repression (silencing) (PubMed : 333393, PubMed : 2128918, PubMed : 8890170, PubMed : 8913298, PubMed : 9398522, PubMed : 16963779, PubMed : 17046956, PubMed : 23543115). Also involved in bacterial chromosome organization and compaction (PubMed : 6379600, PubMed : 10982869, PubMed : 21903814). H-NS binds tightly to AT-rich dsDNA and inhibits transcription (PubMed : 2512122, PubMed : 16963779, PubMed : 17435766, PubMed : 17881364, PubMed : 23543115). Binds upstream and downstream of initiating RNA polymerase, trapping it in a loop and preventing transcription (PubMed : 11714691). Binds to hundreds of sites, approximately half its binding sites are in non-coding DNA, which only accounts for about 10% of the genome (PubMed : 16963779, PubMed : 17046956, PubMed : 23543115). Many of these loci were horizontally transferred (HTG); this offers the selective advantage of silencing foreign DNA while keeping it in the genome in case of need (PubMed : 17046956, PubMed : 17881364, PubMed : 26789284). Suppresses transcription at many intragenic sites as well as transcription of spurious, non-coding RNAs genome-wide (PubMed : 24449106). Repression of HTG by H-NS is tho µght to allow their DNA to evolve faster than non-H-NS-bound regions, and facilitates integration of HTG into transcriptional reg µLatory networks (PubMed : 26789284). A subset of H-NSStpA-reg µLated genes also require Hha (andor Cnu, ydgT) for repression; Hha and Cnu increase the number of genes DNA bound by H-NSStpA and may also mod µLate the oligomerization of the H-NSStpA-complex (PubMed : 23543115). The protein forms 2 clusters in the nucleoid which gather hns-bound loci together, bridging non-contiguous DNA, and causes DNA substantial condensation (PubMed : 21903814). Binds DNA better at low temperatures than at 37 degrees Celsius; AT-rich sites nucleate H-NS binding, further DNA-binding is cooperative and this cooperativity decreases with rising temperature (PubMed : 17435766, PubMed : 17881364). Transcriptional repression can be inhibited by dominant-negative mutants of StpA or itself (PubMed : 8755860). May effect transcriptional elongation (PubMed : 25638302). Can increase translational efficiency of mRNA with suboptimal Shine-Dalgarno sequences (PubMed : 20595230). Plays a role in the thermal control of pili and adhesive curli fimbriae production, by inducing transcription of csgD (PubMed : 17010156). Plays a role in flagellar function (PubMed : 11031114). Represses the CRISPR-cas promoters, permits only weak transcription of the crRNA precursor; its repression is antagonized by LeuO (PubMed : 20132443, PubMed : 20659289). Binds preferentially to the upstream region of its own gene recognizing two segments of DNA on both sides of a bend centered around -150 (PubMed : 7934818). Overexpression suppresses secY24, a temperature-sensitive mutation (PubMed : 1537791). Has also been reported to activate transcription of some genes (PubMed : 4566454, PubMed : 338303, PubMed : 2128918).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : hns
Research Areas : Others
