Volume-based automatic saving-
Buy 5 mL, get $84/ml, or 27% saving
Buy 15 mL, get $70/ml or 39% saving
The unique extension factor, specificity-promoting factors and plateau-inhibiting factor in Phanta Max greatly improve its long-fragment amplification ability, specificity and yield. Phanta Max is capable of amplifying long fragments such as 40 kb λ DNA, 20 kb genomic DNA and 10 kb cDNA. The amplification error rate of Phanta Max is 53-fold lower than that of Taq and 6-fold lower than that of Pfu. In addition,
Phanta Max has a good resistance to PCR inhibitors and can be used for direct PCR amplifications with bacteria, fungi, plants, animal tissues, and even whole blood samples. Phanta Max contains two monoclonal antibodies inhibiting the 5´→3´ polymerase activity and 3´→5´ exonuclease activity at room temperature. Super fidelity and supreme amplification efficiency make Phanta Max the best choice for high fidelity PCR.
2× Phanta Max Master Mix contains Phanta Max Super-Fidelity DNA Polymerase, dNTP, and an optimized buffer system. The amplification can start only with the addition of primer and template, thereby easing PCR setup and improving reproducibility. Protective agents in the 2× Phanta Max Master Mix enable the resistance to repeated freeze-thaw cycles. Amplification will generate blunt-ended products, which are compatible with ClonExpress II One Step Cloning Kit Series (Vazyme, Cat.No. #C112, #C113).

2x GB-AMP™ PaCeR™ HP™ Master Mix is a convenient ready-to-use mixture of GB-AMP™ PaCeR™ HP™ DNA polymerase, dNTP, buffer and magnesium ion. To set up a PCR reaction, you will only need to add the primers, the template and water.
For a brochure on this product, please follow this link.
GB-AMP™ PaCeR™ HP™ DNA polymerase was derived from Pfu DNA Polymerase, after several iterative rounds of protein engineering, the enzyme boasts the on-board unique extension factor, specificity-promoting factors and plateau-inhibiting factor. It is a Fast Pfu-derived polymease.
-Super Fidelity : 53-fold higher than Taq DNA Polymerase and 6-fold better than Pfu DNA polymerase
-Long Range capability : amplify fragments as long as 40 kbλ DNA, 20 kb genomic DNA and 10 kb cDNA
-Diffic µLt PCR suitability : Amplify targets with as high as 85% GC
-Direct-PCR : resists inhibitors in crude lysates of bacteria, fungi, whole blood, c µLtured cells, plant tissues, etc.
-Superior Hot-Start enzyme, employing two MAb as molec µLar thermal switches of polymerase and proof-reading activities.
-Fast PCR : rate of synthesis more than 2x that of Taq DNA polymerase
In an internal comparison studies, GB-AMP™ PaCeR™ HP™ was compared in the following aspects with the leading all-round enzymes such as Phusion (Thermo) and eight other leading DNA polymerases for PCR :
a) Success rate in amplifying human, mouse, rice, wheat, lambda and plasmid DNA, ranging in Volume from 305 to 14768 bp
b) Sensitivity, i.e., ability to detect low copy templates,
c) Speed of amplification with extension time as short as 1 s (for 450 bp) and 5 s (1135 bp)
d) Amplification of High GC template with GC as high as 85%
GB-AMP™ PaCeR™ HP™ was clearly the best all-round enzyme for robustness, sensitivity, speed, high GC amplification and Direct PCR!
A tip on the use : The annealing temperature sho µLd be chosen based on the Tm values of the primers, in general, 55-65°C. See the user manual for details.
The enzyme generates blunt-ended products and thus products can be cloned by our Blunt End Cloning Kits.
Please note : If you are getting a low yield or even no amplification with PaCeR, please consider lowering the annealing temperature by 3-5 °C and/or increasing the annealing temperature (doubling). Talk to our Tech Support as you need.

This product uses the molec µLar-engineered Hot-Start EpiArtTM HS Taq DNA Polymerase and an optimized-buffer system to achieve superior amplification efficiency, sensitivity and specificity for bis µLfite-converted DNA compared with common PCR reagents.
The pre-form µLated 2 × Master Mix can be used for PCR reactions by simply adding primers and templates to reduce the number of pipetting steps and improve thro µghput and the reproducibility of res µLts. EM202 (this product) contains an additional blue dye that is amenable to direct electrophoresis after the reaction is completed, which is thus more convenient to use, than EM 201 (a separate but related product, also available).

This product can be used with the EpiArtTM DNA Methylation Bis µLfite Kit (Vazyme #EM101). The PCR products contain A at 3'-end, which can be directly cloned into the T vector and applied to ClonExpress® Rapid Cloning Kit from GeneBio Systems (Vazyme #C112/C113/C115) and 5 min TA/Blunt-Zero Cloning Kit (Vazyme #C601).

This product uses the molec µLar-engineered Hot-Start EpiArtTM HS Taq DNA Polymerase and an optimized-buffer system to achieve superior amplification efficiency, sensitivity and specificity for bis µLfite-converted DNA compared with common PCR reagents.
The pre-form µLated 2 × Master Mix can be used for PCR reactions by simply adding primers and templates to reduce the number of pipetting steps and improve thro µghput and the reproducibility of res µLts.
A separate and related product, EM202, contains an additional blue dye that is amenable to direct electrophoresis after the reaction is completed, which is thus more convenient to use, than EM 201 (this product)
.
This product can be used with the EpiArtTM DNA Methylation Bis µLfite Kit (Vazyme #EM101). The PCR products contain A at 3'-end, whichcan be directly cloned into the T vector and applied to ClonExpress® Rapid Cloning Kit from GeneBio Systems (Vazyme #C112/C113/C115) and 5 min TA/Blunt-Zero Cloning Kit (Vazyme #C601).

10% OFF list price for the 50ml variant as part of our FALL Sale! On now until October 18th, 2024!
2X Achiever™ Taq Master Mix is a mixture of Taq and Pfu polymerases, blends the processivity of Taq with the high fidelity of Pfu, allowing amplification of the higher fidelity and longer templates than the single-enzyme form µLations. It is also a better choice for amplifying complex template, such as GC-rich and secondary structure-harboring template. And it is suitable as a direct replacement for ordinary Taq Polymerase in most applications. In addition, Using 2X Achiever™ Taq Master Mix res µLts in 3´-dA overhangs PCR products, which can be used in TA clone.
Achiever™ Taq Master Mix is offered in two formats : with and without a blue loading dye. The inclusion of the loading dye means PCR products can be loaded directly onto an agarose gel for analysis. The dye has been validated to not interfere with PCR amplifications. This product described here contains the loading dye (pre-mixed into the 2 x master mix)
If you prefer to add your own loading dye after the completion of PCR, you can select the master mix format without loading dye pre-mixed. Achiever™ Taq Master Mix, Cat. # P211.


Features
Higher fidelity : Its fidelity is four times higher than ordinary Taq polymerase.
High yields : Suitable for complex template which is rich in GC or repeat sequence or template containing secondary structures.
Long PCR : fragments as long as 10 kb from genomic templates and 20 kb from plasmid and phage DNA.
Quality Control
The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests
Functionally tested in PCR

Definition of Activity Unit
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
Store all components at –20°C

Type : Eppendorf tubes, microcentrif µge tubes
Volume : 2 mL
Material : High transparency PP (polypropylene), adapted to microcentrif µges of all models
Resistance : Chemical corrosion and freezing damage
Autoclave Compatibility : Up to 121℃/15 psi
Free of : DNase, RNase, pyrogens
RCF (Relative Centrif µgal Force) : 14,000xg
Low Retention : Interior surface repels water, minimizing retention of solutions
Cap Design : Flat top with a writing surface for positive sample identification; the large cap allows a larger surface area for labeling tubes
Operation : Cap can be opened and closed with one hand; easy to operate
Graduation : Clear graduation markings from 0.1 mL to 2 mL, with intervals of 0.25 mL
Packaging : Each package has a unique lot number, ensuring traceability

Volume : 1000 Tubes
Snap closed and stay closed
Flat top with writing surface for positive sample identification (big cap allows larger surface area for labeling tubes;)
DNAse free/RNAse free.
Made of high transparency PP material, adapted to microcentrif µges of all models
Widely used in molec µLar biological, clinical chemical and biochemical research
Cap can be opened and closed by one hand; easy to operate
Resistant to chemical corrosion and freezing damage
Free of plasticizer and fungistatic, and heavy metal
Stable under high centrif µgal force, up to 15,000rpm can be sustained
Adapted to wide range of temperature from -80 to 121 C, no distortion is observed
Clear graduation on the wise wall for easy observation of liquid volume
Frosted area on cap and on tube wall for convenient marking and labelling
Available in sterilized by E.O.

81 vials
These cardboard storage boxes are designed for the compact storage of 81 each 2ml sample tubes, cryogenic vials, and centrif µge tubes in a temperature of –80℃
100 vials
These cardboard storage boxes are designed for the compact storage of 100 each 2ml sample tubes, cryogenic vials, and centrif µge tubes in a temperature of –80℃
Features :
1. Write-on surface for descriptions or self numbering systems
2. Liquid-resistant coating
3. Designed for use in refrigerators and cryogenic freezer system

Volume 50 Bottles.
250ml transparent PP narrow mouth reagent bottle

Volume : 100 µg
Purification :
Form : liquid
Purity : LT95%
Host : sheep
Clonality : monoclonal
Clone ID :
Isotype :
Storage : PBS with 0.1% Sodium azide at 7.2-7.4
Background :
Immunogen : 25-OH VD
Aliases :
Observed MW :
Uniprot ID :
Reactivity : 25-hydroxy vitamin D2/D3
Application : ELISA
Recommended dilution :
Gene ID :
Research Area :

Volume : 96 Test
Species : Universal
Uniprot ID :
Short name : 25-HVD3
Alias : 25-HVD3|25-Hydroxy Vitamin D3
Sensitivity : 0.469ng/ml
Range : 0.781-50ng/ml
Detection method : Competitive ELISA, Coated with Antigen
Storage_tempeRature : 2-8 °C for 6 months
Research Area :